Douglas Seiler, a research affiliate of the University of California, Berkeley and a co-author of the study, said in a statement about the new method.

Over the past few years, other researchers have digitally opened and decoded some of the scrolls from the Herculaneum villa, which was likely owned by Julius Caesar's father-in-law. These results have helped reveal the writings of ancient philosophers, largely Epicureans and Stoics, with one section even describing Plato's final burial place.

But scientists still wrestled with using X-rays to read the scrolls, since, in many cases, both the papyri and the ink written on them are carbon-based. However, some of these papyri, scientists have learned, contained leaded ink. The new study, published Wednesday (Sept. 16) in the journal PLOS One, suggests that researchers should first scan the scrolls for leaded ink and then use their new technique to read more of these long-lost texts.

This method could help improve text-detection algorithms to identify writing on ancient papyrus pages, they noted. And, in the long run, it may help to protect the scrolls.

"This provides a low-risk method to develop algorithms without endangering the priceless artifacts," the team wrote in the study.

Virtually unrolling a scroll

When Mount Vesuvius erupted in A.D. 79, it buried Herculaneum under 70 feet (20 meters) of volcanic rock and ash.

Along with the 2,000 people who died at Pompeii and Herculaneum from this natural disaster, many of the scrolls housed at the Herculaneum villa were carbonized, or essentially turned into charcoal, trapping their writings in brittle rolls of papyrus that are nearly impossible to read without destroying the scroll itself.

But, given that some of the ink used in the scrolls contained lead, Seiler and his colleagues decided to do a little experiment. They created a fake papyrus scroll with lead-based ink and "carbonized" it to try matching the conditions of the scrolls found at Herculaneum.

Next, the researchers used X-ray fluorescence, which determines the elements in a material by how they interact with X-rays, to determine that these scrolls did, in fact, contain ink with varying levels of lead. Following that, they used X-ray tomography, a way to make a 3D image using X-rays, with custom-made software to re-read some of the text they had produced on their scroll.

Thanks to their technique, the researchers found that they could read the lead-based ink writings in the scrolls, even with minuscule levels of lead. While not all the writings could be deciphered, the method was able to detect differences between the text and the papyrus it was written on.

This technique, if used on other Herculaneum scrolls, could give these ancient writings a "higher probability of being successfully read," they wrote in the study.

How much do you know about the Roman town destroyed by Mount Vesuvius? Find out by taking our Pompeii quiz.

'> New trick could reveal text on 2,000-year-old scrolls charred by eruption of Mount Vesuvius .

In physics, time gets even more complicated, incorporated into theories of relativity — the experience of the clock's ticking being relative to the observer and their place in space — and entropy, which gives time its unidirectional flow. Meanwhile quantum mechanics indicates time can move both forwards and backwards, at least inside quantum systems.

In this excerpt from "On Time: The Physics That Makes the Universe Tick" (Princeton University Press, 2026), author and theoretical physicist Jim Al-Khalili looks at the possibility that time did not begin with the Big Bang — but if this outburst was just another moment, what was there before?

There is an alternative cosmological theory to the one that says the Big Bang marked the beginning of time itself, which was then followed by a brief period of rapid inflation of space. The alternative is known as eternal inflation. In this scenario, our universe was born as a small bubble in an infinite space that has been undergoing inflation forever.

So, rather than time beginning with our Big Bang, we should regard the Big Bang as an event that took place at some moment in time, in which our region of this infinite space suddenly stopped inflating and began instead to expand at a more sedate pace.

So instead of a brief period of inflation happening just after the Big Bang, in the eternal inflation theory it is the other way around, with the Big Bang marking the end of inflation in our part of space, creating our bubble universe outside of which inflation continues. The theory predicts there will be other big bangs happening elsewhere that give rise to other universes — possibly an infinite number of them — all forever separated from each other, and all being driven apart by the ever-expanding, rapidly inflating space outside of them. The totality of this infinite space is referred to as the multiverse, to distinguish it from the individual bubble universes.

Some physicists argue that such an idea falls outside of proper science, since we do not have a way of testing it. There are two other ideas worth mentioning on the matter of whether the Big Bang marked the beginning of time or not, both of which are alternatives to inflation theory. The first is that our universe is part of a repeating process called conformal cyclic cosmology'.

This idea, proposed by Roger Penrose, suggests that our universe is going through an infinite series of lives, or epochs, each of which starts with a big bang and ends with a universe in thermal equilibrium, filled only with evenly spread-out thermal radiation. The idea here is not, as you might expect, that the universe reaches some maximum expansion before collapsing down to a 'Big Crunch' that then marks the Big Bang of the next epoch, but rather because of a subtle geometrical idea called 'conformal mapping' that connects up the very dif­ferent end of one universe (cold and spread-out) with the birth of the next (hot and dense).

I will not say any more about this theory, in particular since it has been argued that it suffers from several flaws, such as the problem of conservation of information (similar to the so-called black hole information paradox) and the difficulty of reconciling the high entropy at the end of the old epoch with the low entropy of the new epoch.

A related hypothesis, which I admit I find aesthetically rather appealing, is known as the ekpyrotic universe model. The name is based on the Greek word ekpyrosis (ἐκπύρωσι), meaning 'conflagration', which refers to a cosmological model proposed by the Stoic philosophers of ancient Greece in which the universe cycles endlessly through fiery birth, expansion, and cooling, followed by contraction, reheating, and rebirth.

The name 'ekpyrotic universe' was devised by one of the modern theory's originators, Paul Steinhardt. The idea comes out of the highly mathematical world of string theory. Basically, the whole of our three-dimensional space (which as we know is really a part of 4D spacetime) forms what is called a 3D brane (a higher-dimensional generalisation of a 2D membrane, or sheet). This 3D brane of ours is floating in a higher-dimensional (4D) space, and the Big Bang is when it collided with a neighbouring brane (in a sense this would be another universe). This collision creates tremendous heat and radiation (the Big Bang) and sets off the expansion of our space.

At the same time, it is drifting away from the other brane that it collided with, but will eventually reach maximum separation from it before they are pulled back together, colliding again in a new Big Bang. Here I simply wish to stress that if this theory is right then there may not have been a beginning of time, and certainly not at the Big Bang.

Another cosmological model, which also does away with the need for inflation, as well as suggesting that the Big Bang was not the beginning of time, has been proposed by another of the originators of the ekpyrotic universe idea, Neil Turok. But this idea, known as the 'mirror universe' theory, is rather dif­ferent. It's a nice idea, and it certainly solves Loschmidt's paradox regarding the conflict between time symmetry and the second law of thermodynamics. Due to the time-symmetric underlying fundamental equations of physics, starting a system off at any arbitrary moment means that its entropy should increase equally into the past as it does into the future.

But this just doesn't make sense. If the system at all times before our chosen arbitrary moment has higher entropy, then starting from any of those earlier times should see the system decrease in entropy in order to reach its state at the original arbitrary moment. Therefore, it cannot be arbitrary; the system had to have been in a specially prepared state at that moment, the moment of the Big Bang. There would be no increase in entropy going backwards in time if that is when time started.

But what if there were a mirror universe on the other temporal side of the Big Bang, evolving in a direction we would consider to be towards the past? This is precisely what Turok is suggesting. What is neat (or convenient, depending on how much you like this theory) is that both sides of the Big Bang are completely separated from each other, with each seeing time pointing in the direction of increasing entropy. If such a mirror universe exists and contains intelligent life that has also hit upon the mirror universe idea, then they would think that our side was the one before the Big Bang in the same way we think that theirs is. As well as doing away with inflation, this idea also conveniently explains why there is more matter than antimatter in our universe because, guess what, the mirror universe would have it the other way round: What they call normal matter is our antimatter.

How seriously should we take these cosmological theories? Are they even proper science? Their proponents know they require some sort of empirical evidence to support their hypotheses, such as some imprint of a reality before the Big Bang frozen in our cosmic microwave background. For without observational evidence, these ideas will remain just theoretical curiosities.

Excerpted from On Time: The Physics That Makes the Universe Tick © 2026 by Jim Al-Khalili. Reprinted by permission of Princeton University Press.

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'> Was there a 'before' the Big Bang? Jim Al-Khalili on the mind-bogglingness of time .

Organoids offer a window into early brain development that's impossible to observe closely in humans, namely because it unfolds inside developing fetuses. Although organoids are not perfect re-creations of full-size human brains, scientists think organoids are useful models for studying the developing brain in both health and disease.

The model in the new study "certainly creates some pretty interesting options in terms of modeling human neurodevelopment and various types of neurodevelopmental disorders," Chen said. "If you're looking for a model that really allows you to look at larger areas of human neural tissue from a cellular, molecular perspective, I think there's a lot that this model has to offer."

Making space for human cells

Often, brain organoids are grown outside living organisms, either in lab dishes or devices that keep these "minibrains" suspended in a solution. Multiple organoids representing different parts of the brain, or even different people's brains, can also be brought together to form more-complex structures.

Why, then, are some scientists growing human organoids inside mice? One reason is that there's a secret sauce inside living organisms that helps organoids mature better in animals than they do in lab dishes. In the body (in vivo), there are mysterious signals that help direct neurons' development and organization, and these signals are missing in lab dishes (in vitro).

"There are some cues that are present in vivo that are really important, and we simply don't know what to add in, in vitro," said study co-author Dr. Sergiu Pașca, a professor of psychiatry and behavioral sciences at Stanford University. The team demonstrated this phenomenon by transplanting organoids into lab rats in a study published in 2022; the transplanted organoids grew larger, formed better connections and were more active than organoids grown in dishes.

But transplanting human brain cells into rodents comes with different challenges. One is that human brains mature at a slower rate than rodent brains do. "Even when they're put in an animal, in a mouse or in a rat, they will still develop about 20 times slower than the mouse or the rat," Pașca told Live Science.

The host's brain cells quickly grow and form new connections, while the human cells lag behind and get outcompeted, Pașca explained. This limits the amount of space that the human cells can take up. As neurons mature, they become myelinated, meaning they gain fatty insulation that helps them communicate more efficiently. The rodent neurons become myelinated more quickly than the human cells do, and that fat creates a physical barrier that the human cells struggle to penetrate, Pașca said.

In their study, described Wednesday (Sept. 16) in the journal Nature, Pașca and colleagues aimed to give the human neurons a leg up. They couldn't solve the issue of human cells maturing slowly, but they could lend the cells extra space to grow in.

Through years of work, they developed a genetically modified mouse that develops only 2% of its cerebral cortex. The majority of its hippocampus — a major memory center in the brain — is also missing. Within a few days of the mouse's birth, the team transplants human neural tissue into that vacant space, and about 90% of the time, that human tissue integrates successfully and starts to grow, Pașca said.

"We just took cortical organoids, about four of them, and transferred them with a syringe into that vacant space," he said. "That's it. They go in there, they graft, and within a few weeks, they start to grow. And then within a few months, they've taken most of that volume."

In the weeks following the transplantation procedure, the human brain cells in the mice's heads grew, formed connections and extended projections into the underlying mouse tissue. The human tissue didn't organize itself into distinct layers as it normally would inside a human's head, but it included many cell types that are typically seen in the human cerebral cortex.

Future of the field

The researchers compared the mice imbued with brain organoids with mice that were missing the same amount of brain tissue but didn't get organoids. They also compared both groups to unmodified lab mice.

Perhaps surprisingly, the mice missing large chunks of their brains still functioned fairly well. "You look at them, and you can't really honestly tell," Pașca said. Upon closer inspection, though, the mice had subtle deficits in their fine-motor skills, working memory and socialization, he noted.

"From our perspective as humans, so much of what we do day to day depends on the cortex," Chen noted. But in a mouse, the cortex makes up a minority of the overall brain, and various studies have suggested that the animals can get by without it, he said. "I don't think the transplantation process itself is significantly hurting the animal," he added.

In the future, the ability to compare these three sets of mice — with organoids, without organoids, and unmodified — could help scientists tease apart how the human tissue is contributing to a given experimental result. Pașca envisions that the approach will be useful for studying the effects of insults to the brain, such as hypoxia (low oxygen) and exposure to toxins or drugs in the womb. Additionally, the genetics of the human organoids could be tweaked to see how those changes affect the brain's development, structure and function. That could be useful for studying cerebral palsy or autism, he suggested.

Chen expects that this new approach will be useful for studying aspects of early brain development at the molecular and cellular levels. The new model incorporates a larger volume of human tissue than models have done in the past, and that's an additional advantage. The human tissue doesn't organize itself into layers or lobes as you'd see in a real human brain, he noted, but he thinks there's still a lot that can be learned from it.

From an ethical standpoint, Pașca consulted with experts at Stanford and an external ethics committee regarding the welfare of the animals used in the study. Studies that involve putting human brain tissue into animals also raise questions about whether that added tissue could grant the animals new cognitive abilities — make them more human-like, in essence. The ethics committee fielded these concerns as well.

"We have not seen emergence of any new properties," Pașca noted. "It's not surprising because we're still at a very early stage of development." They grew the organoids for only six months, so the human organoids were roughly as developed as a 6-month-old fetus's brain tissue.

If the organoids matured to be more human-like — developing layers and lobes — that might present more of a concern. But both Pașca and Chen said that type of development might be hard to recreate in a mouse anyway.

Chen argued that the sheer size of the human brain contributes to its complexity, and it also contains specialized regions that work together to execute different tasks. A mouse's head cannot support the scale of a human brain, and at this point, organoids don't develop the same organization and specialization that we see in people, he said.

"It would be much more problematic if this was to happen in a species that has a larger brain and is closer to humans evolutionarily," Pașca argued. Pigs and nonhuman primates, such as monkeys, would be examples. Especially in regard to transplanting human organoids into monkeys, "that would be an experiment that I don't see is justified at this point," Pașca said.

See how much you know about the most complex organ in the human body with our brain quiz!

'> Scientists shrank mice's brains and replaced the missing tissue with human 'organoids'

"It is extremely rare to witness," study first author Olaf Meynecke, a marine ecologist at Griffith University in Australia, told Live Science in an email. "Only about 20 births of humpback whales have been observed to date and described in literature," none of which involved a stillborn calf.

Unlike the babies of toothed whales and porpoises, newborn humpback whales don't have enough blubber to float, so they sink without the help of an adult whale. This might partly explain why scientists had not witnessed a stillbirth in this species until now, Meynecke said, as the lifeless body sinks to the seabed immediately after birth and is almost impossible to spot from a distance.

The video was taken after study co-author Andrew Mulville, who was on a private boat that morning, noticed two adult humpback whales milling about at the surface of the water. One of the whales adopted a vertical position with its tail pointing up. Moments later, Mulville saw the remains of an amniotic membrane, the inner layer of the placental bag that envelops a fetus, floating on the water.

The whale had obviously just given birth, but there was no sign of the calf. So Mulville and others on the boat followed the female underwater with a GoPro camera. They found the calf dead on the seafloor, while the mother displayed what the study authors interpreted as "postmortem attentive behavior," or grieving.

"She was repeatedly diving down for short times and swimming next to the calf, then resting next to it on the floor for about a minute, even moving the pectoral fin over the calf, then diving up again," Meynecke said. This behavior lasted for at least 90 minutes and possibly days, although the researchers left the scene after about two hours of observation.

The mother did not try to bring the calf's body to the surface, possibly because she realized that it had died during birth. This is different from the postmortem attentive behavior of toothed whales, which researchers have previously seen pushing stillborn babies above the water, according to the study.

The other adult whale on the scene was an "escort" supporting the pregnant female as she prepared to give birth, Meynecke said. Based on the escort's size and appearance, it was likely a female of a similar age to the mom, but the researchers did not confirm this. Male and female escorts are common in humpback whales; they usually travel with females and their calves during migration and assist females during pregnancy.

Grieving behavior suggests that humpback whales have an inherent, strong maternal attachment to their calves. Grief is difficult to demonstrate due to the lack of physiological or neurological measurements. However, the whale mom sought close proximity with her calf during the observation time, and the adult whales were spotted swimming in the area for the next two days, suggesting they were emotionally invested in the death, according to the study.

"It provides a rare window into the behavioural response of baleen [filter-feeding] whales toward a deceased member of their species but also in this case of a mother toward her calf," Meynecke said. "It provides important insight into the complex behaviours of the species and aligns with other observations of care taking, assisting distressed whales or even protecting other species from predators."

'> 'It is extremely rare to witness': Scientists record humpback whale mom grieving her stillborn calf in first-of-its-kind footage

What happened next: Because the man had fainted twice and had a history of heart disease, doctors first looked for problems affecting his brain and heart. An MRI of his brain, used to look for structural abnormalities that could explain the fainting, and an electroencephalogram (EEG), which records the brain's electrical activity, did not reveal a cause.

The man had persistently low blood pressure, the doctors observed, and this led them to move him to the intensive care unit. There, doctors carried out tests for infections and heart attack, but all came back negative.

Meanwhile, the swelling around one of his eyes spread to both eyes and then across his face, while his blood pressure continued to fall despite treatment with intravenous fluids.

The doctors probed further into the man's medical history and activities before the fainting episodes. They learned that he had applied an artificial hair dye two days before he became ill. Soon afterward, he developed itching on his scalp, neck and face. He self-treated with the allergy medicine cetirizine, an antihistamine, for two days. He also reported spending two to three hours outdoors in the sun each day after he'd applied the hair dye.

The diagnosis: Based on the man's recent use of hair dye, the itching that followed, his rapidly worsening facial swelling and his persistently low blood pressure, doctors concluded that he was experiencing anaphylaxis, a severe allergic reaction that can affect the whole body. The reaction can be life-threatening if left untreated.

The treatment: Doctors treated the man with epinephrine (also called adrenaline), a hormonal medication that rapidly reverses the effects of anaphylaxis. They also gave him antihistamines and steroids to help bring the allergic reaction under control, along with intravenous fluids to raise his blood pressure.

However, the patient's blood pressure remained dangerously low, so doctors continued giving him epinephrine through an intravenous drip for about 16 hours. Over the next three days of treatment, his blood pressure stabilized and the swelling in his face gradually subsided.

He was transferred to a general ward and treated there for another five days before being discharged. He was advised that he could have a similar reaction in the future and referred to a dermatologist.

What makes the case unique: Most allergic reactions to hair dye remain limited to the skin, causing itching, redness and swelling where the dye has touched it. Severe reactions affecting the whole body are uncommon, the man's doctors wrote in a report of the case.

The itching the man noticed shortly after applying the dye was likely an early, mild allergic reaction, his doctors wrote. That reaction later progressed to a much more severe one, they suggested, raising the possibility of a "biphasic" allergic reaction, an uncommon pattern seen in about 5% of patients with anaphylaxis. However, this remains only a possible explanation.

The doctors also noted that the man's prolonged sun exposure after he applied the dye may have intensified the reaction; they cited previous research that ultraviolet light can enhance the allergy-triggering effects of para-phenylenediamine, a common ingredient in hair dyes. In the report, the doctors did not identify the exact hair dye ingredient that triggered the reaction in this instance.

The case highlights the importance of asking about recent use of products such as hair dye when doctors evaluate patients with otherwise unexplained fainting, facial swelling or shock, the medical team concluded.

For more intriguing medical cases, check out our Diagnostic Dilemma archives.

This article is for informational purposes only and is not meant to offer medical advice.

Can you guess the diagnosis in these strange medical cases? Find out with our diagnostic dilemma quiz!

'> Diagnostic dilemma: A man's sudden fainting episodes landed him in the hospital — and a hair dye was to blame